MPS Bioinformatics 101: Exploring Massively Parallel Sequencing Data Analysis using the STRait Razor Suite and FDS Tools
Presenters: Jonathan King, Jerry Hoogenboom
Date and Time: Tuesday August 30th, 9 AM – 6 PM
Course Level: This workshop is intended as an introduction for novice users; however, some advanced topics will be discussed.
This workshop will focus on employing the STRait Razor Suite and FDSTools software for analysis of Massively Parallel Sequencing (MPS) data. Attendees will be instructed on the usage of primary (STRait Razor v3 and FDSTools2.0)and secondary analysis (STRait Razor Online) tools to analyze data from commercially available kits. Additionally, guidance will be given for modifying the default settings, developing configuration files for both primary and secondary analysis of laboratory-developed tests (e.g., in-house PCR multiplex assays), and considerations for further processing the output. The strengths of and rationale behind the different methods will be explored by examining the results. This way, attendees will gain a deeper insight into the powers and challenges (and solutions!) that arise from the application of MPS to forensic samples.
Attendees should be familiar with general constructs of DNA markers and formats of standard sequencing data storage file types (e.g., BED, FASTQ, SAM/BAM, etc.). While no formal familiarity with any programming languages(e.g., R, Python, etc.) is required, modification of open-source materials may require some basic understanding of computer programming.